Comparative investigation of the various determinants that influence the codon and amino acid usage patterns in the genus Bifidobacterium

Various strains of the genus Bifidobacterium are crucial members of the human, animal and insect gut, associated with beneficial probiotic activities. An extensive analysis on codon and amino acid usage of the GC rich genus Bifidobacterium has been executed in the present study. Multivariate st...

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Autor principal: Roy, Ayan
Format: Article
Idioma:anglès
Publicat: CrossMark 2025
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Accés en línia:https://repository.auw.edu.bd/handle/123456789/742
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author Roy, Ayan
author_facet Roy, Ayan
author_sort Roy, Ayan
collection institutional Repository
description Various strains of the genus Bifidobacterium are crucial members of the human, animal and insect gut, associated with beneficial probiotic activities. An extensive analysis on codon and amino acid usage of the GC rich genus Bifidobacterium has been executed in the present study. Multivariate statistical analysis revealed a coupled effect of GC compositional constraint and natural selection for translational efficiency to be operative in producing the observed codon usage variations. Gene expression level was inferred to be the most crucial factor governing the codon usage patterns. Amino acid usage was found to be influenced significantly by hydrophobic and aromatic character of the encoded proteins. Gene expressivity and protein energetic cost also had considerable impact on the differential mode of amino acid usage. The genus was found to strictly obey the cost-minimization hypothesis as was reflected from the amino acid usage patterns of the potential highly expressed gene products. Evolutionary analysis revealed that the highly expressed genes were candidates to extreme evolutionary selection pressure and indicated a high degree of conservation at the proteomic level. Interestingly, the complimentary strands of replica- tion appeared to evolve under similar evolutionary constraints which might be addressed as a consequence of absence of replicational selection and lack of strand- specific asymmetry among the members of the genus. Thus, the present endeavor confers considerable know-how pertaining to the codon and amino acid usage intricacies in Bifidobacterium and might prove handy for further scien- tific investigations associated with the concerned domain.
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spelling 123456789-7422026-02-18T06:14:57Z Comparative investigation of the various determinants that influence the codon and amino acid usage patterns in the genus Bifidobacterium Roy, Ayan Bifidobacterium Codon usage Amino acid usage Correspondence analysis Evolutionary rate Various strains of the genus Bifidobacterium are crucial members of the human, animal and insect gut, associated with beneficial probiotic activities. An extensive analysis on codon and amino acid usage of the GC rich genus Bifidobacterium has been executed in the present study. Multivariate statistical analysis revealed a coupled effect of GC compositional constraint and natural selection for translational efficiency to be operative in producing the observed codon usage variations. Gene expression level was inferred to be the most crucial factor governing the codon usage patterns. Amino acid usage was found to be influenced significantly by hydrophobic and aromatic character of the encoded proteins. Gene expressivity and protein energetic cost also had considerable impact on the differential mode of amino acid usage. The genus was found to strictly obey the cost-minimization hypothesis as was reflected from the amino acid usage patterns of the potential highly expressed gene products. Evolutionary analysis revealed that the highly expressed genes were candidates to extreme evolutionary selection pressure and indicated a high degree of conservation at the proteomic level. Interestingly, the complimentary strands of replica- tion appeared to evolve under similar evolutionary constraints which might be addressed as a consequence of absence of replicational selection and lack of strand- specific asymmetry among the members of the genus. Thus, the present endeavor confers considerable know-how pertaining to the codon and amino acid usage intricacies in Bifidobacterium and might prove handy for further scien- tific investigations associated with the concerned domain. 2025-07-22T05:48:16Z 2025-07-22T05:48:16Z 2015 Article https://repository.auw.edu.bd/handle/123456789/742 en application/pdf CrossMark
spellingShingle Bifidobacterium Codon usage Amino acid usage Correspondence analysis Evolutionary rate
Roy, Ayan
Comparative investigation of the various determinants that influence the codon and amino acid usage patterns in the genus Bifidobacterium
title Comparative investigation of the various determinants that influence the codon and amino acid usage patterns in the genus Bifidobacterium
title_full Comparative investigation of the various determinants that influence the codon and amino acid usage patterns in the genus Bifidobacterium
title_fullStr Comparative investigation of the various determinants that influence the codon and amino acid usage patterns in the genus Bifidobacterium
title_full_unstemmed Comparative investigation of the various determinants that influence the codon and amino acid usage patterns in the genus Bifidobacterium
title_short Comparative investigation of the various determinants that influence the codon and amino acid usage patterns in the genus Bifidobacterium
title_sort comparative investigation of the various determinants that influence the codon and amino acid usage patterns in the genus bifidobacterium
topic Bifidobacterium Codon usage Amino acid usage Correspondence analysis Evolutionary rate
url https://repository.auw.edu.bd/handle/123456789/742
work_keys_str_mv AT royayan comparativeinvestigationofthevariousdeterminantsthatinfluencethecodonandaminoacidusagepatternsinthegenusbifidobacterium