Molecular characterization influencing metal resistance in the Cupriavidus/Ralstonia genomes
Our environment is stressed with a load of heavy and toxic metals. Microbes, abundant in our environment are found to adapt well to this metal stressed condition. A comparative study among five Cupriavidus/Ralstonia genomes, can offer a better perception of their evolutionary mechanisms to adapt...
| প্রধান লেখক: | |
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| বিন্যাস: | প্রবন্ধ |
| ভাষা: | ইংরেজি |
| প্রকাশিত: |
CrossMark
2025
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| বিষয়গুলি: | |
| অনলাইন ব্যবহার করুন: | https://repository.auw.edu.bd/handle/123456789/745 |
| _version_ | 1857448963131572224 |
|---|---|
| author | Roy, Ayan |
| author_facet | Roy, Ayan |
| author_sort | Roy, Ayan |
| collection | institutional Repository |
| description | Our environment is stressed with a load of heavy and toxic metals. Microbes, abundant in our
environment are found to adapt well to this metal stressed condition. A comparative study among
five Cupriavidus/Ralstonia genomes, can offer a better perception of their evolutionary
mechanisms to adapt to these conditions. We have studied codon usage among 1051 genes
common to all these organisms and identified 15 optimal codons frequently used in highly
expressed genes present within 1051 genes. We found, the core genes of Cupriavidus
metallidurans CH34 have a different optimal codon choice for Arginine, Glycine and Alanine in
comparison to the other four bacteria. We also found that the synonymous codon-usage bias
within these 1051 core genes is highly correlated with their gene expression. This supports that
translational selection drives synonymous codon usage in the core genes of these genomes.
Synonymous Codon usage is highly conserved in the core genes of these five genomes. The only
exception among them is Cupriavidus metallidurans CH34. This genome-wide shift in
synonymous codon choice in C. metallidurans CH34 may have taken place due to the insertion
of new genes in its genomes facilitating them to survive in heavy-metal containing environment
and the co-evolution of the other genes in its genome to achieve a balance in gene expression.
Structural studies indicated the presence of a longer N-terminal region containing a copper
binding domain in the cupC proteins of C. metallidurans CH3 that helps it to attain higher
binding efficacy with copper in comparison to its orthologs. |
| format | Article |
| id | 123456789-745 |
| institution | Asian University for Women |
| language | English |
| publishDate | 2025 |
| publisher | CrossMark |
| record_format | dspace |
| spelling | 123456789-7452026-02-18T06:15:14Z Molecular characterization influencing metal resistance in the Cupriavidus/Ralstonia genomes Roy, Ayan Core genes, Synonymous codon usage bias, Optimal Codons, Gene expression, Heavy metal resistance, Tertiary structure, Potential energy, Structural stability. Our environment is stressed with a load of heavy and toxic metals. Microbes, abundant in our environment are found to adapt well to this metal stressed condition. A comparative study among five Cupriavidus/Ralstonia genomes, can offer a better perception of their evolutionary mechanisms to adapt to these conditions. We have studied codon usage among 1051 genes common to all these organisms and identified 15 optimal codons frequently used in highly expressed genes present within 1051 genes. We found, the core genes of Cupriavidus metallidurans CH34 have a different optimal codon choice for Arginine, Glycine and Alanine in comparison to the other four bacteria. We also found that the synonymous codon-usage bias within these 1051 core genes is highly correlated with their gene expression. This supports that translational selection drives synonymous codon usage in the core genes of these genomes. Synonymous Codon usage is highly conserved in the core genes of these five genomes. The only exception among them is Cupriavidus metallidurans CH34. This genome-wide shift in synonymous codon choice in C. metallidurans CH34 may have taken place due to the insertion of new genes in its genomes facilitating them to survive in heavy-metal containing environment and the co-evolution of the other genes in its genome to achieve a balance in gene expression. Structural studies indicated the presence of a longer N-terminal region containing a copper binding domain in the cupC proteins of C. metallidurans CH3 that helps it to attain higher binding efficacy with copper in comparison to its orthologs. 2025-07-22T05:50:16Z 2025-07-22T05:50:16Z 2015 Article https://repository.auw.edu.bd/handle/123456789/745 en application/pdf CrossMark |
| spellingShingle | Core genes, Synonymous codon usage bias, Optimal Codons, Gene expression, Heavy metal resistance, Tertiary structure, Potential energy, Structural stability. Roy, Ayan Molecular characterization influencing metal resistance in the Cupriavidus/Ralstonia genomes |
| title | Molecular characterization influencing metal resistance in the Cupriavidus/Ralstonia genomes |
| title_full | Molecular characterization influencing metal resistance in the Cupriavidus/Ralstonia genomes |
| title_fullStr | Molecular characterization influencing metal resistance in the Cupriavidus/Ralstonia genomes |
| title_full_unstemmed | Molecular characterization influencing metal resistance in the Cupriavidus/Ralstonia genomes |
| title_short | Molecular characterization influencing metal resistance in the Cupriavidus/Ralstonia genomes |
| title_sort | molecular characterization influencing metal resistance in the cupriavidus ralstonia genomes |
| topic | Core genes, Synonymous codon usage bias, Optimal Codons, Gene expression, Heavy metal resistance, Tertiary structure, Potential energy, Structural stability. |
| url | https://repository.auw.edu.bd/handle/123456789/745 |
| work_keys_str_mv | AT royayan molecularcharacterizationinfluencingmetalresistanceinthecupriavidusralstoniagenomes |