| Riassunto: | The present study has been aimed to the com-
parative analysis of high GC composition containing
Corynebacterium genomes and their evolutionary study by
exploring codon and amino acid usage patterns. Phyloge-
netic study by MLSA approach, indel analysis and BLAST
matrix differentiated Corynebacterium species in pathogenic
and non-pathogenic clusters. Correspondence analysis on
synonymous codon usage reveals that, gene length, optimal
codon frequencies and tRNA abundance affect the gene
expression of Corynebacterium. Most of the optimal codons
as well as translationally optimal codons are C ending i.e.
RNY (R-purine, N-any nucleotide base, and Y-pyrimidine)
and reveal translational selection pressure on codon bias of
Corynebacterium. Amino acid usage is affected by hydro-
phobicity, aromaticity, protein energy cost, etc. Highly
expressed genes followed the cost minimization hypoth-
esis and are less diverged at their synonymous positions of
codons. Functional analysis of core genes shows significant
difference in pathogenic and non-pathogenic Corynebac-
terium. The study reveals close relationship between non-
pathogenic and opportunistic pathogenic Corynebaterium
as well as between molecular evolution and survival niches
of the organism.
|
|---|