b-sitosterol conjugated silver nanoparticle-mediated amelioration of CCl4-induced liver injury in Swiss albino mice
Objective: Drug induced hepatocyte death is a major contributor to acute liver failure. We aimed to deter- mine whether b-sitosterol conjugated silver nanoparticles (BSAgNPs) could ameliorate carbon tetrachlo- ride (CCl4)-induced liver injury in Swiss albino mice. Methods: Biogenic silver nanop...
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| Format: | Article |
| Language: | English |
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ELSEVIER
2025
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| Online Access: | https://repository.auw.edu.bd/handle/123456789/793 |
| _version_ | 1857449040357097472 |
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| author | Roy, Ayan |
| author_facet | Roy, Ayan |
| author_sort | Roy, Ayan |
| collection | institutional Repository |
| description | Objective: Drug induced hepatocyte death is a major contributor to acute liver failure. We aimed to deter-
mine whether b-sitosterol conjugated silver nanoparticles (BSAgNPs) could ameliorate carbon tetrachlo-
ride (CCl4)-induced liver injury in Swiss albino mice.
Methods: Biogenic silver nanoparticles were synthesized from b-sitosterol to produce b-sitosterol (BS)
conjugated silver nanoparticles. Serum liver function assays in mice model with CCl4-induced liver injury
revealed that alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase
(ALP), bilirubin and cholesterol levels decreased markedly after treatment with b-sitosterol and
BSAgNPs. In vivo liver enzymatic assays, including superoxide dismutase (SOD), catalase and reduced glu-
tathione (GSH) were conducted to assess the antioxidant activity of the treatments.
Results: Liver tissue from BSAgNP treated mice displayed significantly elevated SOD activity (73.57 ± 1.
48%) when compared to positive control group with silymarin treatment. Catalase activity decreased
drastically in CCl4 treated mice (47.14 ± 1.08%), but increased with the administration of BSAgNPs (72.
24 ± 2.25%). An increase in transforming growth factor b (TGF-b1) in liver tissue homogenate accompa-
nied a reduction in nuclear factor erythroid-2-related factor 2 (Nrf2) in CCl4 treated mice. b-sitosterol and
BSAgNPs mediated the reduction of TGF-b1. In the BSAgNPs treated mice, Nrf2 level was significantly ele-
vated; however, no change was detected following b-sitosterol treatment.
Conclusion: Our findings reveal that b-sitosterol conjugated silver nanoparticles (BSAgNPs) may cause
activation of the Nrf2 gene, through potential inhibition of TGF b1/Smad signaling. Antifibrotic effect
of BSAgNPs may promote the lowering of chronic inflammation, oxidative stress and collagen deposition.
Nanoparticle-mediated drug delivery of b-sitosterol may therefore have therapeutic promise against hep-
atic complications. |
| format | Article |
| id | 123456789-793 |
| institution | Asian University for Women |
| language | English |
| publishDate | 2025 |
| publisher | ELSEVIER |
| record_format | dspace |
| spelling | 123456789-7932026-02-18T06:15:03Z b-sitosterol conjugated silver nanoparticle-mediated amelioration of CCl4-induced liver injury in Swiss albino mice Roy, Ayan Objective: Drug induced hepatocyte death is a major contributor to acute liver failure. We aimed to deter- mine whether b-sitosterol conjugated silver nanoparticles (BSAgNPs) could ameliorate carbon tetrachlo- ride (CCl4)-induced liver injury in Swiss albino mice. Methods: Biogenic silver nanoparticles were synthesized from b-sitosterol to produce b-sitosterol (BS) conjugated silver nanoparticles. Serum liver function assays in mice model with CCl4-induced liver injury revealed that alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), bilirubin and cholesterol levels decreased markedly after treatment with b-sitosterol and BSAgNPs. In vivo liver enzymatic assays, including superoxide dismutase (SOD), catalase and reduced glu- tathione (GSH) were conducted to assess the antioxidant activity of the treatments. Results: Liver tissue from BSAgNP treated mice displayed significantly elevated SOD activity (73.57 ± 1. 48%) when compared to positive control group with silymarin treatment. Catalase activity decreased drastically in CCl4 treated mice (47.14 ± 1.08%), but increased with the administration of BSAgNPs (72. 24 ± 2.25%). An increase in transforming growth factor b (TGF-b1) in liver tissue homogenate accompa- nied a reduction in nuclear factor erythroid-2-related factor 2 (Nrf2) in CCl4 treated mice. b-sitosterol and BSAgNPs mediated the reduction of TGF-b1. In the BSAgNPs treated mice, Nrf2 level was significantly ele- vated; however, no change was detected following b-sitosterol treatment. Conclusion: Our findings reveal that b-sitosterol conjugated silver nanoparticles (BSAgNPs) may cause activation of the Nrf2 gene, through potential inhibition of TGF b1/Smad signaling. Antifibrotic effect of BSAgNPs may promote the lowering of chronic inflammation, oxidative stress and collagen deposition. Nanoparticle-mediated drug delivery of b-sitosterol may therefore have therapeutic promise against hep- atic complications. 2025-07-30T03:28:43Z 2025-07-30T03:28:43Z 2022 Article https://repository.auw.edu.bd/handle/123456789/793 en application/pdf ELSEVIER |
| spellingShingle | Roy, Ayan b-sitosterol conjugated silver nanoparticle-mediated amelioration of CCl4-induced liver injury in Swiss albino mice |
| title | b-sitosterol conjugated silver nanoparticle-mediated amelioration of CCl4-induced liver injury in Swiss albino mice |
| title_full | b-sitosterol conjugated silver nanoparticle-mediated amelioration of CCl4-induced liver injury in Swiss albino mice |
| title_fullStr | b-sitosterol conjugated silver nanoparticle-mediated amelioration of CCl4-induced liver injury in Swiss albino mice |
| title_full_unstemmed | b-sitosterol conjugated silver nanoparticle-mediated amelioration of CCl4-induced liver injury in Swiss albino mice |
| title_short | b-sitosterol conjugated silver nanoparticle-mediated amelioration of CCl4-induced liver injury in Swiss albino mice |
| title_sort | b sitosterol conjugated silver nanoparticle mediated amelioration of ccl4 induced liver injury in swiss albino mice |
| url | https://repository.auw.edu.bd/handle/123456789/793 |
| work_keys_str_mv | AT royayan bsitosterolconjugatedsilvernanoparticlemediatedameliorationofccl4inducedliverinjuryinswissalbinomice |