Production of Lysozyme from Duck Egg Page 1 of 26 and Testing its Usefulness in Reduction of Methane Emission in Ruminants
The objectives of this study were to prepare lysozyme from duck egg white and test its usefulness in the reduction of methane emission in ruminants. Triplicates of treatment were added with the additive of lysozyme from duck egg white along with 0.5 g of commercial animal feed and 50 ml of buffer...
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| Format: | Thesis |
| Sprog: | en_US |
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Asian University for Women, Chittagaong, Bangladesh
2022
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| Online adgang: | https://repository.auw.edu.bd/handle/123456789/85 |
| _version_ | 1857448964167565312 |
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| author | Jawaid, Eqra |
| author_facet | Jawaid, Eqra |
| author_sort | Jawaid, Eqra |
| collection | institutional Repository |
| description | The objectives of this study were to prepare lysozyme from duck egg white and test
its usefulness in the reduction of methane emission in ruminants. Triplicates of treatment
were added with the additive of lysozyme from duck egg white along with 0.5 g of
commercial animal feed and 50 ml of buffered rumen fluid. Moreover, controls were added
with 0.5 g of commercial animal feed, 50 ml of buffered rumen fluid but without lysozyme
from duck egg white. Triplicates of treatment and control were set for 24 h and 48 h of
incubation. Total gas (TG) production at different stages was measured for all treatment and
control samples. Total gas produced was also used to measure the amount of CH4 and CO2
released during the incubation period. The optimum incubation period for the experiment (i.e.
24 h and 48 h) was determined after conducting in vitro fertilization for 6 h, 24 h and 48 h of
incubation that followed standard procedures and data was recorded. The data suggested that
there was no production of methane after 6 h of incubation, thus 6 h sampling was dropped
for the actual in vitro experiment. After 24 h of incubation, the mean value of CH4 production
out of total gas production was higher (86.07%) in control as compared to treatment
(76.80%). Similarly, after 48 h of incubation, the mean value of CH4 production out of total
gas production was also higher (55.20%) in control as compared to treatment (50.30%). At
95% confidence interval, there was no significant difference in CH4 reduction in control as
compared to treatment after 24 h and 48 h of incubation. Consequently, the study suggests
that lysozyme from duck egg white supplementation may improve in vitro fermentation and
reduce CH4 emissions from ruminants. |
| format | Thesis |
| id | 123456789-85 |
| institution | Asian University for Women |
| language | en_US |
| publishDate | 2022 |
| publisher | Asian University for Women, Chittagaong, Bangladesh |
| record_format | dspace |
| spelling | 123456789-852026-02-18T06:09:52Z Production of Lysozyme from Duck Egg Page 1 of 26 and Testing its Usefulness in Reduction of Methane Emission in Ruminants Jawaid, Eqra Duck egg white lysozyme, in vitro fermentation, methane, ruminants, global warming The objectives of this study were to prepare lysozyme from duck egg white and test its usefulness in the reduction of methane emission in ruminants. Triplicates of treatment were added with the additive of lysozyme from duck egg white along with 0.5 g of commercial animal feed and 50 ml of buffered rumen fluid. Moreover, controls were added with 0.5 g of commercial animal feed, 50 ml of buffered rumen fluid but without lysozyme from duck egg white. Triplicates of treatment and control were set for 24 h and 48 h of incubation. Total gas (TG) production at different stages was measured for all treatment and control samples. Total gas produced was also used to measure the amount of CH4 and CO2 released during the incubation period. The optimum incubation period for the experiment (i.e. 24 h and 48 h) was determined after conducting in vitro fertilization for 6 h, 24 h and 48 h of incubation that followed standard procedures and data was recorded. The data suggested that there was no production of methane after 6 h of incubation, thus 6 h sampling was dropped for the actual in vitro experiment. After 24 h of incubation, the mean value of CH4 production out of total gas production was higher (86.07%) in control as compared to treatment (76.80%). Similarly, after 48 h of incubation, the mean value of CH4 production out of total gas production was also higher (55.20%) in control as compared to treatment (50.30%). At 95% confidence interval, there was no significant difference in CH4 reduction in control as compared to treatment after 24 h and 48 h of incubation. Consequently, the study suggests that lysozyme from duck egg white supplementation may improve in vitro fermentation and reduce CH4 emissions from ruminants. Submitted by: Eqra Jawaid Advisor: Professor Harunur Rashid Co Advisor: Professor Ashraf Ali Biswas 10/05/2017 2022-12-11T06:04:50Z 2022-12-11T06:04:50Z 2017 Thesis https://repository.auw.edu.bd/handle/123456789/85 en_US application/pdf Asian University for Women, Chittagaong, Bangladesh |
| spellingShingle | Duck egg white lysozyme, in vitro fermentation, methane, ruminants, global warming Jawaid, Eqra Production of Lysozyme from Duck Egg Page 1 of 26 and Testing its Usefulness in Reduction of Methane Emission in Ruminants |
| title | Production of Lysozyme from Duck Egg Page 1 of 26 and Testing its Usefulness in Reduction of Methane Emission in Ruminants |
| title_full | Production of Lysozyme from Duck Egg Page 1 of 26 and Testing its Usefulness in Reduction of Methane Emission in Ruminants |
| title_fullStr | Production of Lysozyme from Duck Egg Page 1 of 26 and Testing its Usefulness in Reduction of Methane Emission in Ruminants |
| title_full_unstemmed | Production of Lysozyme from Duck Egg Page 1 of 26 and Testing its Usefulness in Reduction of Methane Emission in Ruminants |
| title_short | Production of Lysozyme from Duck Egg Page 1 of 26 and Testing its Usefulness in Reduction of Methane Emission in Ruminants |
| title_sort | production of lysozyme from duck egg page 1 of 26 and testing its usefulness in reduction of methane emission in ruminants |
| topic | Duck egg white lysozyme, in vitro fermentation, methane, ruminants, global warming |
| url | https://repository.auw.edu.bd/handle/123456789/85 |
| work_keys_str_mv | AT jawaideqra productionoflysozymefromduckeggpage1of26andtestingitsusefulnessinreductionofmethaneemissioninruminants |