| 要約: | Preparation of various metal nanoparticles using plant extracts has been well studied in recent years. In this study, we found that
nanoparticles synthesized using the extracts of the inforescence of Cocos nucifera exhibited diferential inhibitory activity against
Escherichia coli depending on the nature of the bacterial culture source. Incorporation of silver nanoparticles (Ag-NPs) into the
nutrient broth culture of E. coli resulted in poor inhibitory activity. However, when the silver nanoparticles are added to nutrient
agar plates used for culture of E. coli, efective inhibition was observed. Additionally, E. coli in broth culture resisted the inhibitory
efects of Ag-NPs by forming aggregates of bacterial cells. Te aggregates then generated a protective zone around the colonies to
prevent the entry of Ag-NPs, and the bacterial cells multiplied without inhibition by the Ag-NPs. Tese diferential efects of Ag-
NPs on E. coli culture grown in nutrient broth and on nutrient agar plates indicated that E. coli in broth culture formed aggregates
of cells to develop a bioflm for protection against toxins probably via the quorum sensing mechanism.
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