Culture Method-Dependent Variation in the Sensitivity of Escherichia coli to Silver Nanoparticles

Preparation of various metal nanoparticles using plant extracts has been well studied in recent years. In this study, we found that nanoparticles synthesized using the extracts of the inforescence of Cocos nucifera exhibited diferential inhibitory activity against Escherichia coli depending on the...

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Autor principal: Selvakumar, P. Mosae
Formato: Artigo
Idioma:inglês
Publicado em: Hindawi 2025
Acesso em linha:https://repository.auw.edu.bd/handle/123456789/956
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author Selvakumar, P. Mosae
author_facet Selvakumar, P. Mosae
author_sort Selvakumar, P. Mosae
collection institutional Repository
description Preparation of various metal nanoparticles using plant extracts has been well studied in recent years. In this study, we found that nanoparticles synthesized using the extracts of the inforescence of Cocos nucifera exhibited diferential inhibitory activity against Escherichia coli depending on the nature of the bacterial culture source. Incorporation of silver nanoparticles (Ag-NPs) into the nutrient broth culture of E. coli resulted in poor inhibitory activity. However, when the silver nanoparticles are added to nutrient agar plates used for culture of E. coli, efective inhibition was observed. Additionally, E. coli in broth culture resisted the inhibitory efects of Ag-NPs by forming aggregates of bacterial cells. Te aggregates then generated a protective zone around the colonies to prevent the entry of Ag-NPs, and the bacterial cells multiplied without inhibition by the Ag-NPs. Tese diferential efects of Ag- NPs on E. coli culture grown in nutrient broth and on nutrient agar plates indicated that E. coli in broth culture formed aggregates of cells to develop a bioflm for protection against toxins probably via the quorum sensing mechanism.
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spelling 123456789-9562026-02-18T06:15:27Z Culture Method-Dependent Variation in the Sensitivity of Escherichia coli to Silver Nanoparticles Selvakumar, P. Mosae Preparation of various metal nanoparticles using plant extracts has been well studied in recent years. In this study, we found that nanoparticles synthesized using the extracts of the inforescence of Cocos nucifera exhibited diferential inhibitory activity against Escherichia coli depending on the nature of the bacterial culture source. Incorporation of silver nanoparticles (Ag-NPs) into the nutrient broth culture of E. coli resulted in poor inhibitory activity. However, when the silver nanoparticles are added to nutrient agar plates used for culture of E. coli, efective inhibition was observed. Additionally, E. coli in broth culture resisted the inhibitory efects of Ag-NPs by forming aggregates of bacterial cells. Te aggregates then generated a protective zone around the colonies to prevent the entry of Ag-NPs, and the bacterial cells multiplied without inhibition by the Ag-NPs. Tese diferential efects of Ag- NPs on E. coli culture grown in nutrient broth and on nutrient agar plates indicated that E. coli in broth culture formed aggregates of cells to develop a bioflm for protection against toxins probably via the quorum sensing mechanism. 2025-08-04T13:21:50Z 2025-08-04T13:21:50Z 2023 Article https://repository.auw.edu.bd/handle/123456789/956 en application/pdf Hindawi
spellingShingle Selvakumar, P. Mosae
Culture Method-Dependent Variation in the Sensitivity of Escherichia coli to Silver Nanoparticles
title Culture Method-Dependent Variation in the Sensitivity of Escherichia coli to Silver Nanoparticles
title_full Culture Method-Dependent Variation in the Sensitivity of Escherichia coli to Silver Nanoparticles
title_fullStr Culture Method-Dependent Variation in the Sensitivity of Escherichia coli to Silver Nanoparticles
title_full_unstemmed Culture Method-Dependent Variation in the Sensitivity of Escherichia coli to Silver Nanoparticles
title_short Culture Method-Dependent Variation in the Sensitivity of Escherichia coli to Silver Nanoparticles
title_sort culture method dependent variation in the sensitivity of escherichia coli to silver nanoparticles
url https://repository.auw.edu.bd/handle/123456789/956
work_keys_str_mv AT selvakumarpmosae culturemethoddependentvariationinthesensitivityofescherichiacolitosilvernanoparticles